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Dr. Martin St. Maurice

Dr. Martin St. Maurice
Dr. Martin St. MauriceÌÇÐÄ´«Ã½

Wehr Life Sciences, 210A

MilwaukeeWI53201United States of America
(414) 288-2087

Professor

Structural biology and enzymology

Education

B.Sc., University of Guelph, Canada

Ph.D., Dalhousie University, Canada

Postdoctoral Fellow, University of Wisconsin– Madison

Courses Taught

BIOL 1101 - Foundations in Biological Inquiry

BIOL 1001H - Honors General Biology

BIOL 3101 - Biochemistry and the Molecular Basis for Biology

BIOL 4532 - Biochemistry 2: Bioenergetics and Metabolism

BIOL 4931 - Special Topics in Biology: Current Research in Biochemistry

Research Interests

Enzyme Structure and Function; The Molecular Basis for Catalysis and Regulation in Biotin-dependent Enzymes

Enzymes are the chemical catalysts of biological systems. They are responsible for catalyzing thousands of complex and thermodynamically difficult reactions that are fundamental to all living organisms. Understanding general strategies employed by enzymes and unraveling the molecular interactions in specific enzyme systems continues to advance the fields of medicine and biotechnology. My laboratory works at the exciting interface of chemistry and biology, using the tools of protein engineering, kinetic analyses and X-ray crystallography to determine the structure and function of various enzyme systems at the molecular level. Structural and kinetic analyses, used in tandem, provide a powerful means to probe underlying mechanisms of disease and unveil new targets for therapeutic applications.

Work in my laboratory focuses on understanding the molecular basis for catalysis and allosteric regulation in an important group of metabolic enzymes: the biotin-dependent carboxylases. Dysfunction in these enzymes can lead to genetically inherited disorders that range from benign to severe. In addition, these enzymes offer important targets for the treatment of obesity and type-2 diabetes. The primary goal of my research program is to characterize the mechanism of allosteric control and the molecular basis for catalysis in biotin-dependent carboxylases using X-ray crystallography and steady-state kinetic analyses. One particularly intriguing aspect of catalysis in the biotin-dependent enzyme, pyruvate carboxylase, is the allostery imposed by the activator, acetyl-CoA. This molecule binds asymmetrically to one face of the tetramer and dramatically shifts the orientation and distance between enzyme active sites. My laboratory seeks to clarify the relationship between allosteric regulation and activity in the biotin-dependent carboxylase enzymes with the ultimate goal of unveiling new drug targets and developing molecules with significant therapeutic potential.

Publications

Hakala, J., Laseke, A., Koza, A., St. Maurice, M. (2022) Conformational selection governs carrier domain positioning in Staphylococcus aureus pyruvate carboxylase. Biochemistry, 61:1824–1835.

Schneider, N. O., Tassoulas, L.J., Zeng, D., Laseke, A.J., Reiter, N.J., Wackett, L.P., St. Maurice, M. (2020). Solving the conundrum: widespread proteins annotated for urea metabolism in bacteria are carboxyguanidine deiminases mediating nitrogen assimilation from guanidine. Biochemistry, 59:3258-3270.

Burkett, D. J., Wyatt, B. N., Mews, M., Bautista, A., Engel, R., Dockendorff, C., Donaldson, W. A., St Maurice, M. (2019). Evaluation of α-hydroxycinnamic acids as pyruvate carboxylase inhibitors. Bioorganic and Medicinal Chemistry, 27:4041-4047.

Liu, Y., Budelier, M., Stine, K., St. Maurice, M. (2018). Allosteric regulation alters carrier domain translocation in pyruvate carboxylase. Nature Communications, 9:1384.

Students

Nicholas Schneider (Ph.D. student)
Nneamaka Uba (Ph.D. student)

Dr. St. Maurice is currently accepting new Ph.D. students into his lab

Former Students

Amanda Laseke , Ph.D., 2023
Josh Hakala, Ph.D., 2019
Brittney Wyatt, Ph.D., 2018
Yumeng Liu, Ph.D., 2018
Adam Lietzan, Ph.D., 2014
Yi Lin, Ph.D., 2014


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CONTACT

Department of Biological Sciences

Wehr Life Sciences, 109
1428 W. Clybourn St.
Milwaukee, WI 53233

(414) 288-7355

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